catalog number :
MBS200193
products full name :
beta-Tubulin antibody (clone AT5B2)
products short name :
beta-Tubulin
products name syn :
DKFZp566F223; FLJ98847; MGC8685; Tubulin beta-2B chain; bA506K6.1; TUBB2B; Tubulin beta 2b; Tubulin beta-2B
other names :
tubulin beta-2B chain; Tubulin beta-2B chain; tubulin beta-2B chain; class II beta-tubulin isotype; class IIb beta-tubulin; tubulin, beta polypeptide paralog; tubulin, beta 2B class IIb
other gene names :
TUBB2B; TUBB2B; PMGYSA; bA506K6.1
uniprot entry name :
TBB2B_HUMAN
reactivity :
Human, Mouse
purity :
By protein-G affinity chromatography
form :
Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
storage stability :
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
tested application :
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
app notes :
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:1000 ~ 5000. Recommended starting dilution is 1:1000
other info1 :
Antigen Species: Human. Clone: Anti-human beta-Tubulin mAb, clone AT5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein. Immunogen: Recombinant human beta-Tubulin (1-445aa) purified from E Coli
products categories :
Cytoskeleton
products description :
Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
products references :
Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41(6):746-52. Bouwmeester T, et al. (2004) Nat Cell Bio, 6(2):97-105. Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42(4): 315-330.
ncbi gb acc num :
NM_178012.4
ncbi mol weight :
49,953 Da
ncbi pathways :
Chaperonin-mediated Protein Folding Pathway 106242!!Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway 106243!!Formation Of Tubulin Folding Intermediates By CCT/TriC Pathway 106245!!Gap Junction Pathway 83072!!Gap Junction Pathway 483!!Metabolism Of Proteins Pathway 106230!!Orphan Transporters Pathway 1127546!!Parkin-Ubiquitin Proteasomal System Pathway 700638!!Pathogenic Escherichia Coli Infection Pathway 83103!!Pathogenic Escherichia Coli Infection Pathway 672459
ncbi summary :
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]