MINCLE antibody (AT16E3) | MyBioSource MBS200192 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MINCLE antibody (AT16E3)

catalog :

MBS200192

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT16E3

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200192

products type :

Antibody

products full name :

MINCLE antibody (AT16E3)

products short name :

MINCLE

products name syn :

C-type lectin domain family 4 member E; CLECSF9; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin; CLEC 4E; CLEC4E

other names :

C-type lectin domain family 4 member E; C-type lectin domain family 4 member E; C-type lectin domain family 4 member E; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9; C-type lectin superfamily member 9; macrophage-inducible C-type lectin; C-type lectin domain family 4, member E; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin

products gene name :

MINCLE

other gene names :

CLEC4E; CLEC4E; MINCLE; CLECSF9; CLECSF9; MINCLE

uniprot entry name :

CLC4E_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

AT16E3

host :

Mouse

reactivity :

Human

sequence length :

219

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

app notes :

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

other info1 :

Antigen Species: Human. Clone: Anti-human MINCLE mAb, clone AT16E3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MINCLE protein. Immunogen: Recombinant human MINCLE (41-219aa) purified from E.coli

products categories :

Immunology

products description :

MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.

products references :

Ishikawa E, et al. (2009) J Exp Med, 206(13):2879-88. Matsunaga I, et al. (2009) J Exp Med, 206(13):2865-8. Graham LM, et al. (2009) Cytokine, 48(1-2):148-55.

ncbi gi num :

7657333

ncbi acc num :

NP_055173

ncbi gb acc num :

NM_014358.2

ncbi mol weight :

25,073 Da

ncbi pathways :

Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

uniprot summary :

CLEC4E: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6 -dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to membrane. Molecular Function: receptor activity; carbohydrate binding. Biological Process: immune response; positive regulation of cytokine secretion

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage