Polyclonal Antibody to Cubilin (CUBN) | MyBioSource MBS2001919 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Cubilin (CUBN)

catalog :

MBS2001919

quantity :

0.01 mg

price :

115 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant CUBN, Rat.

image 2 :

DABstainingonIHC-P.Samples:RatTissue)

product information

catalog number :

MBS2001919

products type :

Antibody

products full name :

Polyclonal Antibody to Cubilin (CUBN)

products short name :

[Cubilin (CUBN)]

other names :

[cubilin; Cubilin; cubilin; cubilin; 460 kDa receptor; Glycoprotein 280; gp280; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor]

products gene name :

[CUBN]

other gene names :

[Cubn; Cubn; IFCR; MGA1; GP280; Ifcr; gp280]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Rat

specificity :

The antibody is a rabbit polyclonal antibody raised against CUBN. It has been selected for its ability to recognize CUBN in immunohistochemical staining andwestern blotting.

purity :

Antigen-specific Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Storage : Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.

app notes :

Western blotting: 0.5-2ug/ml. Immunocytochemistry in formalin fixed cells: 5-20ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml. Immunohistochemistry in paraffin section: 5-20ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CUBN (Cys3157~Val3274) expressed in E.coli.

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2065465

ncbi gi num :

16758040

ncbi acc num :

NP_445784.1

ncbi gb acc num :

NM_053332.2

uniprot acc num :

O70244

ncbi mol weight :

398,987 Da

ncbi pathways :

Vitamin Digestion And Absorption Pathway (199553); Vitamin Digestion And Absorption Pathway (199538)

ncbi summary :

receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]

uniprot summary :

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. Chromosomal Location of Human Ortholog: 17q12.3. Cellular Component: apical part of cell; apical plasma membrane; brush border; brush border membrane; clathrin-coated pit; cytoplasm; endocytic vesicle; endoplasmic reticulum; endosome; endosome membrane; Golgi apparatus; lysosomal lumen; lysosomal membrane; membrane; protein complex. Molecular Function: calcium ion binding; cobalamin binding; cobalamin transporter activity; drug binding; hemoglobin binding; identical protein binding; protein binding; protein homodimerization activity; receptor activity. Biological Process: cholesterol metabolic process; cobalamin transport; hemoglobin import; in utero embryonic development; lipoprotein transport; receptor-mediated endocytosis; response to nutrient; vitamin metabolic process

size1 :

0.01 mg

price1 :

115 USD

size2 :

0.02 mg

price2 :

135

size3 :

0.05 mg

price3 :

190

size4 :

0.1 mg

price4 :

245

size5 :

0.2 mg

price5 :

365

size6 :

1 mg

price6 :

870

more info or order :

MyBioSource product webpage