Polyclonal Antibody to Filaggrin 2 (FLG2) | MyBioSource MBS2001794 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Filaggrin 2 (FLG2)

catalog :

MBS2001794

quantity :

0.01 mg

price :

140 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2001794

products type :

Antibody

products full name :

Polyclonal Antibody to Filaggrin 2 (FLG2)

products short name :

Filaggrin 2 (FLG2)

other names :

filaggrin; Filaggrin; filaggrin; epidermal filaggrin; filaggrin

products gene name :

FLG2

other gene names :

FLG; FLG; ATOD2

uniprot entry name :

FILA_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

4061

sequence :

The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-ESQQVESGS TVHGRQETTH GQTINTTRHS QSGQGQSTQT GSRVTRRRRS SQSENSDSEV HSKVSHRHSE HIHTQAGSHY PKSGSTVRRR QGTTHGQRGD TTRHGHSGHG QSTQTGSRTS GRQRFSHSDA TDSEVHSGVS HRPHSQEQ

specificity :

The antibody is a rabbit polyclonal antibody raised against FLG2. It has been selected for its ability to recognize FLG2 in immunohistochemical staining and western blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

200ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:50-400; Immunocytochemistry in formalin fixed cells: 1:50-500; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Immunohistochemistry in paraffin section: 1:10-100; Enzyme-linked Immunosorbent Assay: 1:100-200

other info1 :

Immunogen: Recombinant FLG2 (Glu1222~Gln1368) with N-terminal His-Tag

other info2 :

Quality Control: Content: The quality control contains recombinant CRP (Phe17~Pro224) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control.Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

ncbi gi num :

60097902

ncbi acc num :

NP_002007.1

ncbi gb acc num :

NM_002016.1

uniprot acc num :

P20930

ncbi mol weight :

435,170 Da

ncbi pathways :

AhR Pathway (755436)

ncbi summary :

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

uniprot summary :

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 1q21.3. Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus. Molecular Function: protein binding; calcium ion binding; structural molecule activity. Biological Process: keratinocyte differentiation; multicellular organismal development. Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

255

size3 :

0.1 mg

price3 :

350

more info or order :

MyBioSource product webpage