GAPDH antibody (AT8G4) | MyBioSource MBS200179 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GAPDH antibody (AT8G4)

catalog :

MBS200179

quantity :

0.05 ml

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT8G4

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200179

products type :

Antibody

products full name :

GAPDH antibody (AT8G4)

products short name :

GAPDH

products name syn :

G3PD; GAPD; MGC88685; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PDH; OCAS; p38 component; Glyceraldehyde 3 phosphate dehydrogenase; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; OCT1 coactivator in S phase; 38-KD component; OTTHuMP00000174431; OTTHuMP00000174432; wu:fb33a10.

other names :

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

products gene name :

GAPDH

other gene names :

GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH

uniprot entry name :

G3P_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

AT8G4

host :

Mouse

reactivity :

Human

sequence length :

335

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000. Recommended starting dilution is 1:1,000

other info1 :

Antigen Species: Human. Clone: Anti-human GAPDH mAb, clone AT8G4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GAPDH protein. Immunogen: Recombinant human GAPDH (1-335aa) purified from E.coli

products categories :

Cancer

products description :

Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

products references :

Chuang DM, et al. (2005) Annu Rev Pharmacol Toxicol, 45:269-90. Mazzola JL, et al. (2002) Neurotoxicology, 23(4-5):603-9. Sirover MA. (1997) J Cell Biochem, 66(2):133-40.

ncbi gi num :

7669492

ncbi acc num :

NP_002037

ncbi gb acc num :

NM_002046.5

ncbi mol weight :

31,548 Da

ncbi pathways :

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Alzheimers Disease Pathway 672448!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Disease Pathway 530764!!Gluconeogenesis Pathway 106204!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 413342

ncbi summary :

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

size :

0.05 ml

price :

240 USD

more info or order :

MyBioSource product webpage