antibody

TYMS antibody (clone AT21S5)

MBS200172

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

AT21S5

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

TYMS antibody (clone AT21S5)

TYMS

HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase

thymidylate synthase; Thymidylate synthase; thymidylate synthase; TSase; thymidylate synthetase

TYMS

TYMS; TYMS; TS; TMS; HST422; TS; TS; TSase

TYSY_HUMAN

Monoclonal

IgG2b, k

AT21S5

Mouse

Human

313

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot and Immunofluorescence analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.

Antigen Species: Human. Clone: Anti-human TYMS mAb, clone AT1S5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TYMS protein. Immunogen: Recombinant TYMS (1-313aa) purified from E Coli

Cancer

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in activity highest proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.

Shahrokni A, et al., (2009) Clin Colorectal Cancer 8(4):231-4 Henriquez-Hernandez LA, et al., (2009) Oncol Rep 22(6):1425-33 Colavito D, et al., (2009) Cancer Chemother Pharmacol 64(6):1195-200

4507751

NP_001062

NM_001071.2

26,140 Da

Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); E2F Mediated Regulation Of DNA Replication Pathway (105778); E2F Transcription Factor Network Pathway (137934); Fluoropyrimidine Activity Pathway (198778); G1/S Transition Pathway (105769); G1/S-Specific Transcription Pathway (105776); Integrated Pancreatic Cancer Pathway (711360); Metabolic Pathways (132956); Metabolism Pathway (477135)

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer. Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.1.45; Nucleotide Metabolism - pyrimidine; DNA repair, damage; Mitochondrial; Methyltransferase. Chromosomal Location of Human Ortholog: 18p11.32. Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; cytosol; nucleus. Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding. Biological Process: tetrahydrofolate metabolic process; uracil metabolic process; methylation; circadian rhythm; immortalization of host cell by virus; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; cartilage development; response to cytokine stimulus; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle

0.05 mL

240 USD

0.1 mL

315