G6PD antibody (clone AT2F6) | MyBioSource MBS200169 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

G6PD antibody (clone AT2F6)

catalog :

MBS200169

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2F6

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200169

products type :

Antibody

products full name :

G6PD antibody (clone AT2F6)

products short name :

G6PD

products name syn :

Glucose-6-phosphate 1-dehydrogenase; zwf; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.

other names :

glucose-6-phosphate 1-dehydrogenase isoform b; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase

products gene name :

G6PD

other gene names :

G6PD; G6PD; G6PD1; G6PD

uniprot entry name :

G6PD_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

AT2F6

host :

Mouse

reactivity :

Human

sequence length :

515

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

other info1 :

Antigen Species: Human. Clone: Anti-human G6PD mAb, clone AT2F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human G6PD protein. Immunogen: Recombinant human G6PD (35-506aa) purified from E Coli

products categories :

Metabolism

products description :

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.

products references :

Huang Y., et al, (2008) Mol Genet Metab. 93(1):44-53 Zimny A., et al. (2003) Pol Arch Med Wewn. 110(5):1327-33

ncbi gi num :

108773793

ncbi acc num :

NP_001035810.1

ncbi gb acc num :

NM_001042351.2

ncbi mol weight :

62,468 Da

ncbi pathways :

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Disease Pathway (530764); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Glycogen Storage Diseases Pathway (1127581); Metabolic Pathways (132956)

ncbi summary :

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm. Protein type: Carbohydrate Metabolism - pentose phosphate pathway; Cell development/differentiation; EC 1.1.1.49; Other Amino Acids Metabolism - glutathione; Oxidoreductase. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: centrosome; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; cytosol; nucleus. Molecular Function: identical protein binding; glucose-6-phosphate dehydrogenase activity; protein binding; protein homodimerization activity; NADP binding; glucose binding. Biological Process: NADPH regeneration; response to food; pentose-phosphate shunt; ribose phosphate biosynthetic process; erythrocyte maturation; pentose-phosphate shunt, oxidative branch; glucose 6-phosphate metabolic process; glucose metabolic process; cytokine production; pathogenesis; response to organic cyclic substance; NADP metabolic process; cholesterol biosynthetic process; response to ethanol; substantia nigra development; glutathione metabolic process; carbohydrate metabolic process; regulation of neuron apoptosis; lipid metabolic process; pentose biosynthetic process. Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage