UBE2S antibody (clone AT2C12) | MyBioSource MBS200167 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

UBE2S antibody (clone AT2C12)

catalog :

MBS200167

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2C12

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200167

products type :

Antibody

products full name :

UBE2S antibody (clone AT2C12)

products short name :

UBE2S

products name syn :

E2-EPF; E2EPF; EPF5; ubiquitin-conjugating enzyme E2S AA409170; MGC101982; uBE2S; ubiquitin carrier protein S; ubiquitin protein ligase S; ubiquitin conjugating enzyme E2 24 kD; ubiquitin conjugating enzyme E2S

other names :

ubiquitin-conjugating enzyme E2 S; Ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2 S; E2-EPF5; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-protein ligase S; ubiquitin-conjugating enzyme E2S; E2-EPF; Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S

products gene name :

UBE2S

other gene names :

UBE2S; UBE2S; EPF5; E2EPF; E2-EPF; E2EPF

uniprot entry name :

UBE2S_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, k

clone :

AT2C12

host :

Mouse

reactivity :

Human

sequence length :

222

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250

other info1 :

Antigen Species: Human. Clone: Anti-human UBE2S mAb, clone AT2C12, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UBE2S protein. Immunogen: Recombinant human UBE2S (1-222aa) purified from E Coli

products categories :

Ubiquitination

products description :

UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.

products references :

Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10(11):755-64. Windheim M, et al. (2008) Biochem J, 409(3):723-9.

ncbi gi num :

112382377

ncbi acc num :

NP_055316.2

ncbi gb acc num :

NM_014501.2

ncbi mol weight :

23,845 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

uniprot summary :

UBE2S: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes Lys-11 -linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating Lys-11 -linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except Lys-48 -linked polyubiquitination. Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL. Belongs to the ubiquitin-conjugating enzyme family. Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 19q13.43. Cellular Component: anaphase-promoting complex; cytoplasm. Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity. Biological Process: anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; protein modification process; exit from mitosis

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage