antibody

PSMD11 antibody (clone AT1F4)

MBS200160

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

AT1F4

human, mouse

western blot, ELISA, enzyme immunoassay

Antibody

PSMD11 antibody (clone AT1F4)

PSMD11

proteasome (prosome; macropain) 26S subunit non-ATPase 11; S9; p44.5; MGC3844; Rpn6

26S proteasome non-ATPase regulatory subunit 11; 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome non-ATPase regulatory subunit 11; 26S proteasome regulatory subunit 9; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p44.5; proteasome (prosome, macropain) 26S subunit, non-ATPase, 11; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit p44.5

PSMD11

PSMD11; PSMD11; S9; Rpn6; p44.5

PSD11_HUMAN

Monoclonal

IgG1, k

AT1F4

Mouse

Human, Mouse

422

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1000. Recommended starting dilution is 1:250.

Antigen Species: Human. Clone: Anti-human PSMD11 mAb, clone AT1F4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PSMD11 protein. Immunogen: Recombinant human PSMD11 (1-422aa) purified from E Coli

Ubiquitination

PSMD11 (Proteasome 26S non-ATPase subunit 11), also known as S9, Rpn6 or p44.5, has multi-subunit protease complexes consisting of 20S subunits composed of four seven-numbered rings with two outer rings containing alpha subunits and two central rings composed of beta subunits, and 19S caps of 6 ATPase and 11 non-ATPase subunits. PSMD11 is the main proteolytic enzyme that functions in ATP-dependent degradation of ubiquitinated proteins.

Sato Y, et al., (2009) Biochem Biophys Res Commun. 378(4):795-8 Shibahara T, et al., (2002) Eur J Biochem. 269(5):1474-83

28872725

NP_002806

NM_002815.3

47,535 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway (1127621); APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway (1127616); APC Truncation Mutants Have Impaired AXIN Binding Pathway (1127617); APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway (1127549); AUF1 (hnRNP D0) Destabilizes MRNA Pathway (187212); AXIN Missense Mutants Destabilize The Destruction Complex Pathway (1127620)

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

PSMD11: Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Belongs to the proteasome subunit S9 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Proteasome complex. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: nucleoplasm; proteasome complex; membrane; proteasome regulatory particle; nucleus; cytosol. Molecular Function: protein binding. Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; stem cell differentiation; proteasome assembly; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

0.05 mL

240 USD

0.1 mL

315