BRCC36 antibody (clone AT3B1) | MyBioSource MBS200155 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

BRCC36 antibody (clone AT3B1)

catalog :

MBS200155

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT3B1

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200155

products type :

Antibody

products full name :

BRCC36 antibody (clone AT3B1)

products short name :

BRCC36

products name syn :

BRCA1/BRCA2-containing complex subunit 3; chromosome X open reading frame 53; C6.1A

other names :

lys-63-specific deubiquitinase BRCC36 isoform 1; Lys-63-specific deubiquitinase BRCC36; lys-63-specific deubiquitinase BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCA1/BRCA2-containing complex, subunit 3; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36

products gene name :

BRCC36

other gene names :

BRCC3; BRCC3; C6.1A; BRCC36; CXorf53; BRCC36; C6.1A; CXorf53

uniprot entry name :

BRCC3_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

AT3B1

host :

Mouse

reactivity :

Human

sequence length :

316

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

other info1 :

Antigen Species: Human. Clone: Anti-human BRCC36 mAb, clone AT3B1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BRCC36 protein. Immunogen: Recombinant BRCC36 (1-316aa) purified from E Coli

products categories :

Ubiquitination

products description :

BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

products references :

Wang Bin, et al., (2007) Proc Natl Acad Sci USA 104(52):20759-20763 Chen Xiaowei, et al., (2006) Cancer Research 66:5039-5046 Dong Y, et al., (2003) Mol Cell 12(5):1087-1099

ncbi gi num :

13236583

ncbi acc num :

NP_077308

ncbi gb acc num :

NM_024332.3

ncbi mol weight :

28,027 Da

ncbi summary :

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]

uniprot summary :

BRCC3: Metalloprotease that specifically cleaves Lys-63 - linked polyubiquitin chains. Does not have activity toward Lys- 48 -linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes Lys-63 -linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes Lys-63 -linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double- strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves Lys-63 -linked ubiquitin in various substrates. Mediates the specific Lys-63 - specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA. Belongs to the peptidase M67A family. BRCC36 subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system; EC 3.4.19.-; Oncoprotein; Ubiquitin-specific protease. Chromosomal Location of Human Ortholog: Xq28. Cellular Component: nucleoplasm; nuclear ubiquitin ligase complex; nucleus; ubiquitin ligase complex. Molecular Function: protein binding; metallopeptidase activity; metal ion binding; ubiquitin-specific protease activity; polyubiquitin binding; enzyme regulator activity. Biological Process: positive regulation of DNA repair; regulation of catalytic activity; double-strand break repair; response to ionizing radiation; G2/M transition DNA damage checkpoint; proteolysis; response to X-ray

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage