catalog number :
MBS200144
products full name :
Monoclonal anti-ORF73/HHV8 antibody (clone AT4C11). Mouse IgG 2a ,K
products short name :
ORF73/HHV8
products name syn :
Human herpesvirus 8 ORF73; Kaposi's sarcoma-associated herpesvirus
other names :
ORF 73; ORF 73; ORF 73
products gene name :
ORF73/HHV8
uniprot entry name :
O40947_HHV8
isotype :
Mouse IgG 2a heavy chain and kappa light chain
purity :
By protein-G affinity chromatography
form :
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
storage stability :
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
tested application :
ELISA (EIA), Western Blot (WB) (Cell lysate; transfected), Immunohistochemistry (IHC)
app notes :
The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
other info1 :
Antigen Species: Human. Clone: Anti-human ORF73/HHV8 mAb, clone AT4C11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ORF73/HHV8 protein. Immunogen: Recombinant human ORF73/HHV8 (122-329aa) purified from E Coli
products categories :
Neuroscience
products description :
ORF73 protein of human herpesvirus 8(HHV-8) is a kaposi's sarcoma-associated herpesvirus (kSHV) latent protein which has been suspected of playing a crucial role in modulating viral and cellular gene expression. ORF73 is associated with cellular chromatin and stays on the chromosomes during cell division. It maintains the viral genomes during cell division by tethering the viral episomes to the chromosomes. It binds directly to replication origin recognition complexes (ORCs) that are primarily associated with the terminal repeat (TR) region of the HHV-8 genome.
products references :
kedes D.H., et al. (1997) Journal of Clinical Investigation. 100: 2610-2606. Verma SC., et al (2006) Journal of Virology 80: 3445-3458.
ncbi mol weight :
126,233 Da