EPHX1 antibody (clone AT2E5) | MyBioSource MBS200142 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

EPHX1 antibody (clone AT2E5)

catalog :

MBS200142

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2E5

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200142

products type :

Antibody

products full name :

EPHX1 antibody (clone AT2E5)

products short name :

EPHX1

products name syn :

epoxide hydrolase 1; epoxide hydrolase 1 microsomal (xenobiotic)

other names :

epoxide hydrolase 1; Epoxide hydrolase 1; epoxide hydrolase 1; epoxide hydratase; epoxide hydrolase 1, microsomal (xenobiotic); Epoxide hydratase; Microsomal epoxide hydrolase

products gene name :

EPHX1

other gene names :

EPHX1; EPHX1; MEH; EPHX; EPOX; HYL1; EPHX; EPOX

uniprot entry name :

HYEP_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, k

clone :

AT2E5

host :

Mouse

reactivity :

Human, Mouse

sequence length :

455

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Tissue)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

other info1 :

Antigen Species: Human. Clone: Anti-human EPHX1 mAb, clone AT2E5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EPHX1 protein. Immunogen: Recombinant human EPHX1 (21-455aa) purified from E Coli

products categories :

Signal Transduction

products description :

Epoxide hydrolase 1 (EPHX1) is a family of biotransformation enzyme which is involved in the hydrolysis of various epoxides and epoxide intermediates. Epoxide hydrolase plays an important role in both the activation and detoxification of exogenous chemicals such as polycyclic aromatic hydrocarbons.

products references :

Bertvan Loo, et al., (2006) Appl Environ Microbiol 72(4):2905-2917 Janet k.C, et al., (2001) The Histochemical Journal 33:329-336 Hassett C, et al., (1994) Genomics 23(2):433-442

ncbi gi num :

4503583

ncbi acc num :

NP_000111

ncbi gb acc num :

NM_000120.3

ncbi mol weight :

52,949 Da

ncbi pathways :

Aflatoxin B1 Metabolism Pathway (198808); Benzo(a)pyrene Metabolism Pathway (198911); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (425); Metapathway Biotransformation (198837)

ncbi summary :

Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]

uniprot summary :

Ephx1: Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls. Defects in EPHX1 are a cause of familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the peptidase S33 family. Protein type: Hydrolase; Xenobiotic Metabolism - metabolism by cytochrome P450; Membrane protein, integral; EC 3.3.2.9. Chromosomal Location of Human Ortholog: 1q42.1. Cellular Component: endoplasmic reticulum membrane; integral to membrane. Molecular Function: cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity. Biological Process: response to toxin; aromatic compound catabolic process; response to organic cyclic substance. Disease: Hypercholanemia, Familial; Preeclampsia/eclampsia 1

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage