Monoclonal Antibody to Apolipoprotein A1 (APOA1) | MyBioSource MBS2001346 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Apolipoprotein A1 (APOA1)

catalog :

MBS2001346

quantity :

0.01 mg

price :

100 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[B3]

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Human Serum; Primary Ab: 2ug/ml Mouse Anti-Human APOA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

image 2 :

Western Blot: Sample: RecombinantAPOA1,Human.

image 3 :

DAB staining on IHC-P; Samples: Human Kidney Tissue.

product information

catalog number :

MBS2001346

products type :

Antibody

products full name :

Monoclonal Antibody to Apolipoprotein A1 (APOA1)

products short name :

[Apolipoprotein A1 (APOA1)]

other names :

[apolipoprotein A-I isoform 1 preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apolipoprotein A1; Apolipoprotein A1]

products gene name :

[APOA1]

other gene names :

[APOA1; APOA1; apo(a); Apo-AI; ApoA-I; ProapoA-I]

clonality :

Monoclonal

isotype :

IgG

clone :

[B3]

host :

Mouse

reactivity :

Human

sequence length :

sequence :

Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-QPYLDDFQK KWQEEMELYR QKVEPLRAEL QEGARQKLHE LQEKLSPLGE EMRDRARAHV DALRTHLAPY SDELRQRLAA RLEALKENGG ARLAEYHAKA

specificity :

The antibody is a mouse monoclonal antibody raised against APOA1. It has been selected for its ability to recognize APOA1 in immunohistochemical staining andwestern blotting.

purity :

Affinity Chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

concentration :

500ug/ml

storage stability :

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

app notes :

Western blotting: 1:100-400. Immunocytochemistry in formalin fixed cells: 1:100-500. Immunohistochemistry in formalin fixed frozen section: 1:100-500. Immunohistochemistry in paraffin section: 1:50-200. Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Human Liver Tissue

image5 heading :

Immunohistochemistry (IHC)

image5 description :

DAB staining on IHC-P; Samples: Human Breast Cancer Tissue

other info1 :

Immunogen: APOA1, Human. Immunogen Information: Recombinant APOA1 (Gln122~Gln267) with N-terminal His-Tag expressed in E.coli. Accession No.: RPA519Hu01

other info2 :

Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2043260

ncbi gi num :

4557321

ncbi acc num :

NP_000030.1

ncbi gb acc num :

NM_000039.2

uniprot acc num :

P02647

ncbi mol weight :

30,778 Da

ncbi pathways :

ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

ncbi summary :

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

uniprot summary :

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family. Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle. Chromosomal Location of Human Ortholog: 11q23.3. Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; cytosol; early endosome; endocytic vesicle; endoplasmic reticulum lumen; extracellular matrix; extracellular region; extracellular space; nucleus; plasma membrane. Molecular Function: amyloid-beta binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; heat shock protein binding; high-density lipoprotein particle binding; identical protein binding; lipase inhibitor activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity; protein binding. Biological Process: adrenal gland development; axon regeneration in the peripheral nervous system; blood vessel endothelial cell migration; cellular protein metabolic process; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; G-protein coupled receptor signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipid storage; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion involved in immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; neuron projection regeneration; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid efflux; phospholipid homeostasis; platelet degranulation; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; post-translational protein modification; protein oxidation; protein stabilization; receptor-mediated endocytosis; regulation of Cdc42 protein signal transduction; regulation of intestinal cholesterol absorption; regulation of lipid metabolic process; regulation of protein amino acid phosphorylation; response to drug; response to estrogen; response to nutrient; retinoid metabolic process; reverse cholesterol transport; transforming growth factor beta receptor signaling pathway; transmembrane transport; triglyceride catabolic process; vitamin transport. Disease: Amyloidosis, Familial Visceral; Hypoalphalipoproteinemia, Primary

size1 :

0.01 mg

price1 :

100 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

140

size4 :

0.1 mg

price4 :

170

size5 :

0.2 mg

price5 :

220

size6 :

1 mg

price6 :

495

more info or order :

MyBioSource product webpage