ATGL antibody (clone AT2G2) | MyBioSource MBS200134 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

ATGL antibody (clone AT2G2)

catalog :

MBS200134

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT2G2

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200134

products type :

Antibody

products full name :

ATGL antibody (clone AT2G2)

products short name :

ATGL

products name syn :

patatin-like phospholipase domain containing 2; nuclear factor of activated T-cells; cytoplasmic; calcineurin-dependent 1; NF-ATC; NFATc; NFAT2; Adipose triglyceride lipase; FP17548; TTS-2.2; desnutrin; PNPLA2

other names :

patatin-like phospholipase domain-containing protein 2; Patatin-like phospholipase domain-containing protein 2; patatin-like phospholipase domain-containing protein 2; IPLA2-zeta; TTS2.2; adipose triglyceride lipase; calcium-independent phospholipase A2; desnutrin; patatin-like phospholipase domain containing protein 2; pigment epithelium-derived factor; transport-secretion protein 2.2; triglyceride hydrolase; patatin-like phospholipase domain containing 2; Adipose triglyceride lipase; Calcium-independent phospholipase A2; Desnutrin; IPLA2-zeta; Pigment epithelium-derived factor; TTS2.2; Transport-secretion protein 2; TTS2

products gene name :

ATGL

other gene names :

PNPLA2; PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik; ATGL; TTS2

uniprot entry name :

PLPL2_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

AT2G2

host :

Mouse

reactivity :

Human, Mouse

sequence length :

504

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Tissue), Immunohistochemistry (IHC)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

other info1 :

Antigen Species: Human. Clone: Anti-human ATGL mAb, clone AT2G2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATGL protein. Immunogen: Recombinant human ATGL (30-504aa) purified from E Coli

products categories :

Enzymes & Proteases

products description :

Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

products references :

Fischer J, et al., (2007) Nat Genet 39(1):28-30 Elena S, et al., (2006) EMBO Rep 7(1):106-113 Zimmermann R, et al., (2004) Science 306(5700):1383-1386

ncbi gi num :

32698724

ncbi acc num :

NP_065109

ncbi gb acc num :

NM_020376.3

ncbi mol weight :

19,875 Da

ncbi pathways :

Acyl Chain Remodeling Of DAG And TAG Pathway (645315); Acylglycerol Degradation Pathway (413388); Acylglycerol Degradation Pathway (468272); Fatty Acid Beta Oxidation Pathway (198865); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycerophospholipid Biosynthesis Pathway (645313); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]

uniprot summary :

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Lipase; EC 3.1.1.3. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; lipid particle; cytosol. Molecular Function: triacylglycerol lipase activity. Biological Process: sequestering of lipid; phospholipid metabolic process; glycerophospholipid biosynthetic process; triacylglycerol catabolic process. Disease: Neutral Lipid Storage Disease With Myopathy

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage