antibody

NFATc1 antibody (clone AT1C3)

MBS200132

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

AT1C3

western blot, ELISA, enzyme immunoassay

Antibody

NFATc1 antibody (clone AT1C3)

NFATc1

nuclear factor of activated T-cells; cytosolic component 1 isoform A; NF-ATC; NFATc; NFAT2

nuclear factor of activated T-cells, cytoplasmic 1 isoform A; Nuclear factor of activated T-cells, cytoplasmic 1; nuclear factor of activated T-cells, cytoplasmic 1; NFAT transcription complex cytosolic component; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1; NFAT transcription complex cytosolic component; NF-ATc; NFATc

NFATc1

NFATC1; NFATC1; NFAT2; NFATc; NF-ATC; NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc

NFAC1_HUMAN

Monoclonal

IgG2a, k

AT1C3

Mouse

Human

716

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

Antigen Species: Human. Clone: Anti-human NFATc1 mAb, clone AT1C3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NFATc1 protein. Immunogen: Recombinant human NFATc1 (428-716aa) purified from E Coli

Transcription & Translation

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1(NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.

Akimzhanov A, et al., (2008) Am J Pathol 172(1):215-224 Asagiri M, et al., (2005) J Exp Med 202(9):1261-1269 Rao A, et al., (1997) Annu Rev Immunol 15:707-747

27502393

NP_765978

NM_172390.2

38,626 Da

B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Ca2+ Pathway (1127532); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); FCERI Mediated Ca+2 Mobilization Pathway (833827); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825)

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 18q23. Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; cytosol; nucleus. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; FK506 binding; mitogen-activated protein kinase p38 binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; calcium ion transport; epithelial to mesenchymal transition; innate immune response; positive regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; G1/S transition of mitotic cell cycle

0.05 mL

240 USD

0.1 mL

315