catalog number :
MBS2001317
products full name :
Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)
products short name :
S100 Calcium Binding Protein A9 (S100A9)
other names :
S100 calcium binding protein A9; Protein S100-A9; protein S100-A9; MRP-14; calgranulin B; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B); S100 calcium binding protein A9; Calgranulin-B; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
products gene name :
S100A9
other gene names :
S100A9; S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387; CAGB; CFAG; MRP14; MRP-14; p14
uniprot entry name :
S10A9_HUMAN
specificity :
The antibody is a mouse monoclonal antibody raised against S100A9. It has been selected for its ability to recognize S100A9 in immunohistochemical staining and western blotting.
purity :
Protein A/G Affinity Chromatography.
form :
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
app notes :
Western blotting: 0.5-5ug/ml. Immunocytochemistry in formalin fixed cells: 5-30ug/ml. Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml. Immunohistochemistry in paraffin section: 5-30ug/ml. Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Optimal working dilutions must be determined by end user.
other info1 :
Source: Monoclonal antibody preparation. Traits: Liquid
other info2 :
Immunogen: Recombinant S100A9 (Met1~Pro114) expressed in E.coli.
ncbi acc num :
AAH47681.1
ncbi summary :
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]
uniprot summary :
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cytoskeleton; extracellular region; plasma membrane; nucleus; cytosol. Molecular Function: arachidonic acid binding; antioxidant activity; RAGE receptor binding; signal transducer activity; protein binding; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; defense response to bacterium; actin cytoskeleton reorganization; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response