Monoclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5) | MyBioSource MBS2001302 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Monoclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5)

catalog :

MBS2001302

quantity :

0.01 mg

price :

105 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[D10]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Recombinant ALOX5, Human.

image 2 :

Western Blot; Sample: Human Placenta lysate;. Primary Ab: 29g/ml Mouse Anti-Human ALOX5 Antibody. Second Ab: 0.29g/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody. (Catalog: MBS2086035)

product information

catalog number :

MBS2001302

products type :

Antibody

products full name :

Monoclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5)

products short name :

[Arachidonate-5-Lipoxygenase (ALOX5)]

other names :

[arachidonate 15-lipoxygenase; Arachidonate 15-lipoxygenase; arachidonate 15-lipoxygenase; arachidonate 15-lipoxygenase; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31]

products gene name :

[ALOX5]

other gene names :

[ALOX15; ALOX15; LOG15; 12-LOX; 15-LOX; 15-LOX-1; LOG15; 12-LOX]

clonality :

Monoclonal

isotype :

IgG2b Kappa

clone :

[D10]

host :

Mouse

purity :

Protein A + Protein G affinity chromatography

form :

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300,50% glycerol.

concentration :

1mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 24 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2 ug/mL. Immunohistochemistry: 5-20 ug/mL. Immunocytochemistry: 5-20 ug/mL. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

other info1 :

Organism Species: Homo sapiens (Human). Source: Monoclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant 5-LO (Glu461~Ser671) expressed in E.coli

ncbi gi num :

40316937

ncbi acc num :

NP_001131.3

ncbi gb acc num :

NM_001140.4

uniprot acc num :

P16050

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (685553); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); IL4-mediated Signaling Events Pathway (137933); Linoleic Acid Metabolism Pathway (82992); Linoleic Acid Metabolism Pathway (367); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

uniprot summary :

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family. Protein type: EC 1.13.11.31; EC 1.13.11.33; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; Oxidoreductase. Chromosomal Location of Human Ortholog: 17p13.2. Cellular Component: cytosol; extrinsic component of cytoplasmic side of plasma membrane; lipid droplet; membrane; plasma membrane. Molecular Function: arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding. Biological Process: apoptotic cell clearance; arachidonic acid metabolic process; bone mineralization; cellular response to calcium ion; cellular response to interleukin-13; hepoxilin biosynthetic process; inflammatory response; leukotriene metabolic process; lipoxin A4 biosynthetic process; lipoxygenase pathway; negative regulation of adaptive immune response; ossification; phosphatidylethanolamine biosynthetic process; positive regulation of actin filament polymerization; positive regulation of cell-substrate adhesion; positive regulation of ERK1 and ERK2 cascade; positive regulation of heterotypic cell-cell adhesion; regulation of engulfment of apoptotic cell; wound healing

size1 :

0.01 mg

price1 :

105 USD

size2 :

0.02 mg

price2 :

110

size3 :

0.05 mg

price3 :

145

size4 :

0.1 mg

price4 :

175

size5 :

0.2 mg

price5 :

230

size6 :

1 mg

price6 :

515

more info or order :

MyBioSource product webpage