Cofilin 1 antibody (clone AT1C1) | MyBioSource MBS200127 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Cofilin 1 antibody (clone AT1C1)

catalog :

MBS200127

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT1C1

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200127

products type :

Antibody

products full name :

Cofilin 1 antibody (clone AT1C1)

products short name :

Cofilin 1

products name syn :

CFL1; CFL; Cofilin 1; CFL1; CFL; 18 kDa phosphoprotein(p18) 18 kDa phosphoprotein; CFL 1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; P18.

other names :

cofilin-1; Cofilin-1; cofilin-1; 18 kDa phosphoprotein; cofilin, non-muscle isoform; epididymis secretory protein Li 15; p18; cofilin 1 (non-muscle); 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform

other gene names :

CFL1; CFL1; CFL; HEL-S-15; CFL; p18

uniprot entry name :

COF1_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

AT1C1

host :

Mouse

reactivity :

Human

sequence length :

166

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Tissue, Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-human Cofilin 1 mAb, clone AT1C1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cofilin 1 protein. Immunogen: Recombinant human Cofilin 1 (1-166aa) purified from E Coli

products categories :

Cytoskeleton

products description :

Cofilin is a family of actin-binding proteins which disassembles actin filaments. It is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

products references :

Mannherz H.G., et al. (2007) J Mol Biol 366(3):745-755 Olsen J.V., et al. (2006) Cell 127(3):635-648 Yang N., et al. (1998) Nature 393(6687):809-812

ncbi gi num :

5031635

ncbi acc num :

NP_005498

ncbi gb acc num :

NM_005507.2

ncbi mol weight :

18,502 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); CDC42 Signaling Events Pathway (137994); CXCR4-mediated Signaling Events Pathway (137910); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1127691); EPHB-mediated Forward Signaling Pathway (1127693); Fc Gamma R-mediated Phagocytosis Pathway (114228)

ncbi summary :

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

uniprot summary :

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; cortical actin cytoskeleton; nuclear matrix; focal adhesion; membrane; cytoplasm; intercellular junction; nucleus; vesicle. Molecular Function: protein binding; actin binding. Biological Process: actin filament depolymerization; axon guidance; platelet activation; positive regulation of actin filament depolymerization; response to virus; cytoskeleton organization and biogenesis; negative regulation of cell size; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; cytokinesis after mitosis; establishment of cell polarity; protein import into nucleus; ephrin receptor signaling pathway; innate immune response; regulation of cell morphogenesis; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage