antibody

FLIP antibody (clone 5D8)

MBS200126

0.05 ml

240 USD

monoclonal

mouse

nonconjugated

5D8

human, mouse, rat

western blot, ELISA, enzyme immunoassay

Antibody

FLIP antibody (clone 5D8)

FLIP

CASP8 and FADD-like apoptosis regulator isoform 1; CFLAR; CASH; CASP8AP1; CLARP; MRIT; Caspase homolog; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; Cellular FLICE-like inhibitory protein; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity

CASP8 and FADD-like apoptosis regulator isoform 1; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator; FADD-like antiapoptotic molecule 1; MACH-related inducer of toxicity; caspase homolog; caspase-eight-related protein; caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; inhibitor of FLICE; usurpin beta; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein; CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; UsurpinCleaved into the following 2 chains:CASP8 and FADD-like apoptosis regulator subunit p43; CASP8 and FADD-like apoptosis regulator subunit p12

FLIP

CFLAR; CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1; CASH; CASP8AP1; CLARP; MRIT; CASH; Casper; CLARP; c-FLIP; FLAME-1; I-FLICE; MRIT

CFLAR_HUMAN

Monoclonal

IgG3, k

5D8

Mouse

Human, Mouse, Rat

480

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Tissue, Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Antigen Species: Human. Clone: Anti-human FLIP mAb, clone 5D8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FLIP protein. Immunogen: Recombinant human FLIP (1-376aa) purified from E Coli

Apoptosis

FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (death receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (tumor necrosis factor receptor 1).

Yu JW, Shi Y., (2008) Oncogene. 27(48):6216-27 Du C, et al., (2005) kidney Int. 67(4):1397-409

187608571

NP_003870

NM_003879.5

44,112 Da

Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822!!Caspase-8 Activation By Cleavage Pathway 105654!!Chagas Disease (American Trypanosomiasis) Pathway 147809!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Death Receptor Signalling Pathway 105650!!Dimerization Of Procaspase-8 Pathway 105655

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

0.05 ml

240 USD