antibody

EphA2 antibody (clone 3D7)

MBS200110

0.05 ml

240 USD

monoclonal

mouse

nonconjugated

3D7

human, mouse

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

EphA2 antibody (clone 3D7)

EphA2

Ephrin receptor EphA2; epoxide hydrolase 1 microsomal (xenobiotic)

ephrin type-A receptor 2; Ephrin type-A receptor 2; ephrin type-A receptor 2; epithelial cell receptor protein tyrosine kinase; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; EPH receptor A2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK

EphA2

EPHA2; EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6; ECK

EPHA2_HUMAN

Monoclonal

IgG2b, k

3D7

Mouse

Human, Mouse

976

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:100.

Antigen Species: Human. Clone: Anti-human EphA2 mAb, clone 3D7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human EphA2 protein. Immunogen: Recombinant human EphA2 (559-976aa) purified from E Coli

Enzymes & Proteases

The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk). Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.

Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5(3), 149-57 Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59(1), 58-67

32967311

NP_004422

NM_004431.3

54,305 Da

Arf6 Signaling Events Pathway 138034!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Direct P53 Effectors Pathway 137939!!EPH-Ephrin Signaling Pathway 1127691!!EPH-ephrin Mediated Repulsion Of Cells Pathway 1127695!!EPHA Forward Signaling Pathway 138041!!EPHA-mediated Growth Cone Collapse Pathway 1127692

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

0.05 ml

240 USD