antibody

Nanog antibody (clone5A10)

MBS200100

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

5A10

human, mouse

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Nanog antibody (clone5A10)

Nanog

Nanog homeobox; FLJ12581; FLJ40451

homeobox protein NANOG isoform 1; Homeobox protein NANOG; homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Homeobox transcription factor Nanog; hNanog

NANOG

NANOG; NANOG; hNanog

NANOG_HUMAN

Monoclonal

IgG2a, k

5A10

Mouse

Human, Mouse

305

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500~1:1,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:50.

Antigen Species: Human. Clone: Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein. Immunogen: Recombinant human Nanog (1-154aa) purified from E Coli

Immunology

Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Tay Y., et al. (2008) Nature. 455(7216):1124-8 Chiou SH., et al. (2008) Clin Cancer Res. 14(13):4085-95 Boer B., et al. (2008) Mol Reprod Dev.

153945816

NP_079141

NM_024865.3

32,838 Da

Cardiac Progenitor Differentiation Pathway (712094); Developmental Biology Pathway (477129); POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway (1127697); POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway (1127698); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); SIDS Susceptibility Pathways (198901); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Regulation Of Pluripotent Stem Cells Pathway (1127696)

The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

NANOG: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5 -TAAT[GT][GT]-3 or 5 -[CG][GA][CG]C[GC]ATTAN[GC]-3 . When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Cell development/differentiation; DNA-binding. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: nucleoplasm; nucleolus; nucleus. Molecular Function: DNA binding; chromatin binding; transcription factor activity; transcription corepressor activity. Biological Process: gonad development; response to retinoic acid; transcription, DNA-dependent; somatic stem cell maintenance; stem cell division; endodermal cell fate specification; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; positive regulation of mitotic cell cycle; mesodermal cell fate commitment; negative regulation of BMP signaling pathway; negative regulation of cell fate commitment; cell proliferation; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

0.05 mL

240 USD

0.1 mL

315