TARDBP antibody (clone k1B8) | MyBioSource MBS200097 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TARDBP antibody (clone k1B8)

catalog :

MBS200097

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1B8

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200097

products type :

Antibody

products full name :

TARDBP antibody (clone k1B8)

products short name :

TARDBP

products name syn :

TAR DNA binding protein; TDP-43; ALS10; TAR DNA-binding protein 43; TDP43

other names :

TAR DNA-binding protein 43; TAR DNA-binding protein 43; TAR DNA-binding protein 43; TAR DNA-binding protein-43; TAR DNA binding protein

products gene name :

TARDBP

other gene names :

TARDBP; TARDBP; ALS10; TDP-43; TDP43; TDP-43

uniprot entry name :

TADBP_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

k1B8

host :

Mouse

reactivity :

Human

sequence length :

414

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

other info1 :

Antigen Species: Human. Clone: Anti-human TARDBP mAb, clone k1B8, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TARDBP protein. Immunogen: Recombinant human TARDBP (1-260aa) purified from E Coli

products categories :

Transcription & Translation

products description :

TAR DNA binding protein (TARDBP), also known as TDP-43, is a cellular protein. TARDBP was identified as the disease accumulating protein in patients with frontotemporal lobar degeneration (FTLD) with ubiquitin inclusions (FTLD-U) and in amyotrophic lateral sclerosis (ALS). TARDBP functions as a DNA-binding protein and specifically binds to the TAR DNA sequence motifs of HIV. Via this association with TAR motifs, TARDBP acts as a transcriptional repressor and inhibits HIV-1 transcription. In addition, this protein regulates alternate splicing of the cystic fibrosis transmembrane receptor (CFTR) gene.

products references :

Nicola JR, et al., (2008) PLoS Genet 4(9):e1000193 kabashi E, et al., (2008) Nat Genet 40(5):572-574 Ou SH, et al., (1995) J Virol 69:3584-3596

ncbi gi num :

6678271

ncbi acc num :

NP_031401

ncbi gb acc num :

NM_007375.3

ncbi mol weight :

31,808 Da

ncbi summary :

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

uniprot summary :

TARDBP: DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3 -splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3 UTR. Homodimer. Binds specifically to pyrimidine-rich motifs of TAR DNA and to single stranded TG repeated sequences. Binds to RNA, specifically to UG repeated sequences with a minimun of six contiguous repeats. Interacts with ATNX2; the interaction is RNA-dependent. Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. 3 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: nucleoplasm; perichromatin fibrils; cytoplasm; nuclear speck; nucleus. Molecular Function: identical protein binding; protein binding; mRNA 3 -UTR binding; RNA binding; double-stranded DNA binding; nucleotide binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; RNA splicing; positive regulation of insulin secretion; positive regulation of transcription from RNA polymerase II promoter; mRNA processing. Disease: Amyotrophic Lateral Sclerosis 10, With Or Without Frontotemporal Dementia

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage