PPP1R14A antibody (clone 4H10) | MyBioSource MBS200095 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PPP1R14A antibody (clone 4H10)

catalog :

MBS200095

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4H10

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200095

products type :

Antibody

products full name :

PPP1R14A antibody (clone 4H10)

products short name :

PPP1R14A

products name syn :

CPI17; CPI-17; PPP1INL; Protein phosphatase 1; regulatory (inhibitor) subunit 14A; PPP1R14A; Protein phosphatase 1; regulatory (inhibitor) subunit 14A CPI 17; CPI 17 alpha; CPI 17alpha; CPI17; PKC potentiated inhibitory protein of PP1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A

other names :

protein phosphatase 1 regulatory subunit 14A isoform 1; Protein phosphatase 1 regulatory subunit 14A; protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of 17 kDa; protein phosphatase 1, regulatory (inhibitor) subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17

products gene name :

PPP1R14A

other gene names :

PPP1R14A; PPP1R14A; CPI17; CPI-17; PPP1INL; CPI17; PPP1INL; CPI-17

uniprot entry name :

PP14A_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, k

clone :

4H10

host :

Mouse

reactivity :

Human

sequence length :

147

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info1 :

Antigen Species: Human. Clone: Anti-human PPP1R14A mAb, clone 4H10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1R14A protein. Immunogen: Recombinant human PPP1R14A (1-147aa) purified from E Coli

products categories :

Signal Transduction

products description :

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.

products references :

Hamaguchi., et al. (2000) Biochem. Biophys. Res Commun. 274(3):825-30 Eto M., et al. (1997) FEBS Lett. 410(2-3):356-60 Lartey I., et al. (2007) Biol Reprod. 76(6):971-82

ncbi gi num :

15147250

ncbi acc num :

NP_150281

ncbi gb acc num :

NM_033256.2

ncbi mol weight :

13,480 Da

ncbi pathways :

Vascular Smooth Muscle Contraction Pathway (96530); Vascular Smooth Muscle Contraction Pathway (96866)

ncbi summary :

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A. Protein type: Inhibitor; Protein phosphatase, regulatory subunit. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: cytoplasm. Molecular Function: protein phosphatase inhibitor activity. Biological Process: regulation of phosphorylation; negative regulation of catalytic activity

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage