IL33 antibody (clone 4E9) | MyBioSource MBS200087 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IL33 antibody (clone 4E9)

catalog :

MBS200087

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4.00E+09

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200087

products type :

Antibody

products full name :

IL33 antibody (clone 4E9)

products short name :

IL33

products name syn :

Interleukin 33; DVS27; NF-HEV; NKHEV; C9orf26; IL-33; Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26; DKFZp586H0523; DVS27 related protein; IL 1F11; IL 33; IL1F11; Interleukin 1 family member 11; INTERLEuKIN 33 NFHEV; Interleukin 33 precursor; Interleukin33; NF HEV; NFHEV; Nuclear factor from high endothelial venules; RP11 575C20.2; il33

other names :

interleukin-33 isoform 1; Interleukin-33; interleukin-33; DVS27-related protein; IL-1F11; IL-33; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules; interleukin 33; Interleukin-1 family member 11; IL-1F11; Nuclear factor from high endothelial venules; NF-HEV

products gene name :

IL33

other gene names :

IL33; IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV

uniprot entry name :

IL33_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

4.00E+09

host :

Mouse

reactivity :

Human

sequence length :

270

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info1 :

Antigen Species: Human. Clone: Anti-human IL-33 mAb, clone 4E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-33 protein. Immunogen: Recombinant human IL-33 (112-270aa) purified from E Coli

products categories :

Hormones & Cytokines

products description :

Interleukin 33 (IL-33) is a 32kDa proinflammatory cytokine and intracellular nuclear factor with transcriptional regulatory properties. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.

products references :

Brint Ek, et al., (2005) J Biol Chem. 20:277(51) Schmitz, et al., (2005) immunity. 23:479 Baekkevold ES, et al., (2003) Am.J.Pathol. 163(1):69-90

ncbi gi num :

15559209

ncbi acc num :

NP_254274

ncbi gb acc num :

NM_033439.3

ncbi mol weight :

16,250 Da

ncbi pathways :

Cytosolic DNA-sensing Pathway (125137); Cytosolic DNA-sensing Pathway (124833); Influenza A Pathway (217173); Influenza A Pathway (217150)

ncbi summary :

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]

uniprot summary :

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 9p24.1. Cellular Component: extracellular space; transport vesicle; chromosome; nucleus. Molecular Function: protein binding; cytokine activity. Biological Process: positive regulation of T-helper 2 type immune response; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage