antibody

PDCD4 antibody (clone k4C1)

MBS200081

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

k4C1

western blot, ELISA, enzyme immunoassay

Antibody

PDCD4 antibody (clone k4C1)

PDCD4

neoplastic transformation inhibitor; H731; MGC33046; MGC33047; Programmed cell death 4 isoform 1; PDPC4; Programmed cell death 4 isoform 1; PDCD4; programmed cell death 4 (neoplastic transformation inhibitor); nuclear antigen H731

programmed cell death protein 4 isoform 1; Programmed cell death protein 4; programmed cell death protein 4; neoplastic transformation inhibitor protein; nuclear antigen H731; protein 197/15a; programmed cell death 4 (neoplastic transformation inhibitor); Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a

PDCD4

PDCD4; PDCD4; H731; H731

PDCD4_HUMAN

Monoclonal

IgG1, k

k4C1

Mouse

Human

469

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 3,000. Recommended starting dilution is 1:2,000.

Antigen Species: Human. Clone: Anti-human PDCD4 mAb, clone k4C1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCD4 protein. Immunogen: Recombinant human PDCD4 (1-469aa) purified from E Coli

Cancer

Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

Palamarchuk A, et al., (2006) Cancer Res 65(24):11282-11286 Yoshina, et al., (1999) Pathol Int 49(12):1067-1077

21735596

NP_055271

NM_014456.4

50,576 Da

MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); MTOR Signaling Pathway (138001)

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified. Protein type: Tumor suppressor; Apoptosis. Chromosomal Location of Human Ortholog: 10q24. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; RNA binding. Biological Process: negative regulation of JNK activity; apoptosis; cell aging; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle

0.05 mL

240 USD

0.1 mL

315