Visfatin antibody (clone 4D5) | MyBioSource MBS200079 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Visfatin antibody (clone 4D5)

catalog :

MBS200079

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4D5

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200079

products type :

Antibody

products full name :

Visfatin antibody (clone 4D5)

products short name :

Visfatin

products name syn :

Pre-B-cell colony-enhancing factor 1; NAMPT; MGC117256; Nicotinamide phosphoribosyltransferase precursor; PBEF; PBEF1; VF; NAMPT; Visfatin; Nicotinamide phosphoribosyltransferase precursor; pre-B-cell colony enhancing factor 1

other names :

nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; nicotinamide phosphoribosyltransferase; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

other gene names :

NAMPT; NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik; PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor

uniprot entry name :

NAMPT_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

4D5

host :

Mouse

reactivity :

Human

sequence length :

491

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-human Visfatin mAb, clone 4D5, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Visfatin protein. Immunogen: Recombinant human Visfatin (1-491aa) purified from E Coli

products categories :

Hormones & Cytokines

products description :

Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor.

products references :

Stephens JM, et al., (2006) Curr Opin Lipidol. 17:128-31 Hug C, et al., (2005) Science. 307:366-367

ncbi gi num :

5031977

ncbi acc num :

NP_005737

ncbi gb acc num :

NM_005746.2

ncbi mol weight :

55,521 Da

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003)

ncbi summary :

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

uniprot summary :

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. Protein type: EC 2.4.2.12; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation; Transferase; Cytokine. Chromosomal Location of Human Ortholog: 7q22.3. Cellular Component: extracellular space; nucleus; cytosol. Molecular Function: protein binding; protein homodimerization activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; cytokine activity; drug binding; nicotinamide phosphoribosyltransferase activity. Biological Process: circadian rhythm; NAD biosynthetic process; vitamin metabolic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; female pregnancy; circadian regulation of gene expression; signal transduction; response to organic cyclic substance; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage