CD73 antibody (clone 2B6) | MyBioSource MBS200071 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD73 antibody (clone 2B6)

catalog :

MBS200071

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2B6

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200071

products type :

Antibody

products full name :

CD73 antibody (clone 2B6)

products short name :

CD73

products name syn :

5' nucleotidase; ecto; 5'-nucleotidase ecto (CD73); 5' nucleotidase (CD73); CD73; eN; eNT

other names :

5'-nucleotidase isoform 1 preproprotein; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; 5'-nucleotidase, ecto (CD73); Ecto-5'-nucleotidase; CD_antigen: CD73

products gene name :

CD73

other gene names :

NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; NT5; NTE; 5'-NT

uniprot entry name :

5NTD_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

2B6

host :

Mouse

reactivity :

Human

sequence length :

574

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-human CD73 mAb, clone 2B6, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein. Immunogen: Recombinant human CD73 (27-252aa) purified from E Coli

products categories :

Immunology

products description :

CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

products references :

Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360 Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405 Laura A, et al., (1997) J Cell Biol 136(2):421-431

ncbi gi num :

4505467

ncbi acc num :

NP_002517

ncbi gb acc num :

NM_002526.3

ncbi mol weight :

57,949 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

uniprot summary :

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, GPI anchor; Membrane protein, integral; EC 3.1.3.5. Chromosomal Location of Human Ortholog: 6q14-q21. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; nucleotide binding. Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of inflammatory response; adenosine biosynthetic process; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process. Disease: Calcification Of Joints And Arteries

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage