Rab5a antibody (clone 3A4) | MyBioSource MBS200066 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rab5a antibody (clone 3A4)

catalog :

MBS200066

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A4

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200066

products type :

Antibody

products full name :

Rab5a antibody (clone 3A4)

products short name :

Rab5a

products name syn :

RAB5A; member RAS oncogene family; RAB5; Rab5a; RAB5A; member RAS oncogene family RAB 5; RAB 5A; RAB5A member RAS oncogene family; RAS associated protein RAB5A; Ras related protein Rab 5A.

other names :

ras-related protein Rab-5A isoform 1; Ras-related protein Rab-5A; ras-related protein Rab-5A; RAS-associated protein RAB5A; RAB5A, member RAS oncogene family

products gene name :

Rab5a

other gene names :

RAB5A; RAB5A; RAB5; RAB5

uniprot entry name :

RAB5A_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

3A4

host :

Mouse

reactivity :

Human, Mouse

sequence length :

215

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info1 :

Antigen Species: Human. Clone: Anti-human Rab5a mAb, clone 3A4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Rab5a protein. Immunogen: Recombinant human Rab5a (1-215aa) purified from E Coli

products categories :

Cancer

products description :

Rab5a is a member of the RAS oncogene family. Rab5a is a small GTPase localized to the plasma membrane and early endosomes. It is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex.

products references :

Bucci C, et al., (1992) Cell 70 (5): 715-28 Seachrist JL, et al., (2002) J. Biol. Chem. 277 (1): 679-85

ncbi gi num :

19923262

ncbi acc num :

NP_004153

ncbi gb acc num :

NM_004162.4

ncbi mol weight :

22,178 Da

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279); Endocytosis Pathway (102181); ErbB1 Downstream Signaling Pathway (138057); Factors Involved In Megakaryocyte Development And Platelet Production Pathway (187196)

uniprot summary :

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein, monomeric, Rab; G protein, monomeric; Motility/polarity/chemotaxis; G protein. Chromosomal Location of Human Ortholog: 3p24-p22. Cellular Component: zymogen granule membrane; protein complex; dendrite; early endosome; terminal button; phagocytic vesicle; cytosol; actin cytoskeleton; lipid raft; ruffle; synaptic vesicle; recycling endosome; cell soma; axon; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; positive regulation of smooth muscle cell migration; blood coagulation; Rab protein signal transduction; positive regulation of exocytosis

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage