antibody

Hexokinase antibody (clone 4D7)

MBS200057

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

4D7

western blot, ELISA, enzyme immunoassay

Antibody

Hexokinase antibody (clone 4D7)

Hexokinase

HK1; Hexokinase 1; EC 2.7.1.1; Hexokinase type I; HK I; Brain form hexokinase; Hexokinase-1; BB404130; dea; Glycolytic enzyme; HEXOKIN; Hexokinase PI; Hexokinase type I; Hexokinase; tumor isozyme; Hexokinase-A; HK1 tb; Hk1-s; HK1-ta; HK1-tc; HKI; HXK1; mHk1-s.

hexokinase-1 isoform HKI; Hexokinase-1; hexokinase-1; HK I; brain form hexokinase; glycolytic enzyme; hexokinase type I; hexokinase 1; Brain form hexokinase; Hexokinase type I; HK I

HK1; HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc; HK I

HXK1_HUMAN

Monoclonal

IgG2a, k

4D7

Mouse

Human

917

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.

Antigen Species: Human. Clone: Anti-human Hexokinase1 mAb, clone 4D7, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase1 protein. Immunogen: Recombinant human Hexokinase1 (1-917aa) purified from E Coli

Signal Transduction

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this hexokinase1 have been associated with hemolytic anemia due to hexokinase deficiency.

Ellison W R. et al., (1975) J Biol Chem 250:1864-1871 Furuta H. et al., (1996) Genomics 36(1):206-9 Jon E. et al., (2003) J.Exp Biology 206:2049-2057

188497754

NP_000179

NM_000188.2

101,085 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Butirosin And Neomycin Biosynthesis Pathway (145809); Butirosin And Neomycin Biosynthesis Pathway (145786); Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231)

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the Warburg effect . Acts as a glucose sensor by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells. Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Mitochondrial; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.1; Carbohydrate Metabolism - galactose; Kinase, other; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - starch and sucrose. Chromosomal Location of Human Ortholog: 10q22. Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft. Molecular Function: hexokinase activity; protein binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding. Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport. Disease: Hemolytic Anemia, Nonspherocytic, Due To Hexokinase Deficiency; Neuropathy, Hereditary Motor And Sensory, Russe Type

0.05 mL

240 USD

0.1 mL

315