UNG antibody (clone k1C12) | MyBioSource MBS200056 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

UNG antibody (clone k1C12)

catalog :

MBS200056

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

k1C12

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human	Svilar D, Dyavaiah M, Brown A, Tang J, Li J, McDonald P, et al. Alkylation sensitivity screens reveal a conserved cross-species functionome. Mol Cancer Res. 2012;10:1580-96 pubmed publisher

product information

catalog number :

MBS200056

products type :

Antibody

products full name :

UNG antibody (clone k1C12)

products short name :

UNG

products name syn :

uracil-DNA glycosylase isoform UNG2; uracil-DNA glycosylase; UDG; UNG1; UNG2; HIGM4; uracil-DNA glycosylase 1; uracil-DNA glycosylase 2

other names :

uracil-DNA glycosylase isoform UNG2; Uracil-DNA glycosylase; uracil-DNA glycosylase; UDG; UNG; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; uracil-DNA glycosylase

products gene name :

UNG

other gene names :

UNG; UNG; DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15;

uniprot entry name :

UNG_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

k1C12

host :

Mouse

reactivity :

Human

sequence length :

313

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-human UNG mAb, clone k1C12, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UNG protein. Immunogen: Recombinant human UNG (1-313aa) purified from E Coli

products categories :

Hormones & Cytokines

products description :

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species.

products references :

Jau der C et al.,(1991) J Bacteriol 173(1):283-290 Haug T et al.,(1998) Nucleic Acids Res. 26(6):1449-1457 Hilde N et al.,(2000) Nucleic Acids Res. 28(12):2277-2285 Stuart R.W. Bellamy et al.,(2007) Nucleic Acids Res. 35(5):1478-1487 Chih chung L et al.,(2007) J Virol 81(3):1195-1208

ncbi gi num :

19718751

ncbi acc num :

NP_550433

ncbi gb acc num :

NM_080911.2

ncbi mol weight :

33,924 Da

ncbi pathways :

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Pyrimidine Pathway (105845); DNA Repair Pathway (105837); Depyrimidination Pathway (105843); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Primary Immunodeficiency Pathway (83125)

ncbi summary :

This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]

uniprot summary :

UNG: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections. Belongs to the uracil-DNA glycosylase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.2.2.27; Hydrolase. Chromosomal Location of Human Ortholog: 12q23-q24.1. Cellular Component: nucleoplasm; mitochondrion; nucleus. Molecular Function: protein binding; uracil DNA N-glycosylase activity. Biological Process: viral reproduction; base-excision repair, AP site formation; depyrimidination; base-excision repair; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; DNA repair; negative regulation of apoptosis. Disease: Immunodeficiency With Hyper-igm, Type 5

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage