MBP antibody (clone 10C12) | MyBioSource MBS200053 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MBP antibody (clone 10C12)

catalog :

MBS200053

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10C12

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200053

products type :

Antibody

products full name :

MBP antibody (clone 10C12)

products short name :

MBP

products name syn :

Maltose binding protein

other names :

MBP, partial; Myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein

products gene name :

MBP

other gene names :

MBP; MBP; MBP

uniprot entry name :

MBP_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

10C12

host :

Mouse

reactivity :

Human

sequence length :

160

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Recombinant protein)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.

other info1 :

Antigen Species: Human. Clone: Anti-MBP mAb, clone 10C12, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant MBP protein. Immunogen: Recombinant MBP purified from E Coli

products categories :

Protein Marker

products description :

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

products references :

Jonathan D et al.,(1996) Proc Natl Acad Sci USA 93:211-215 Weiming Y et al., (2001) EMBO J 20(3):362-371 Chen Z et al., (2005) Proc Natl Acad Sci USA 102(29):10200-10205

ncbi gi num :

49456793

ncbi acc num :

CAG46717.1

uniprot acc num :

P02686

ncbi mol weight :

17,343 Da

ncbi pathways :

Glial Cell Differentiation Pathway (698758); MAPK Cascade Pathway (198834); Neural Crest Differentiation Pathway (672460); Spinal Cord Injury Pathway (739007)

ncbi summary :

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]

uniprot summary :

MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 18q23. Cellular Component: compact myelin; internode region of axon; cell soma; plasma membrane; nucleus. Molecular Function: protein binding; structural constituent of myelin sheath; protease binding. Biological Process: myelination; response to mercury ion; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; immune response; negative regulation of axonogenesis; axon ensheathment; response to progesterone stimulus; aging

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage