Hsp27 antibody (clone 2A5) | MyBioSource MBS200046 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Hsp27 antibody (clone 2A5)

catalog :

MBS200046

quantity :

0.05 ml

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2A5

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200046

products type :

Antibody

products full name :

Hsp27 antibody (clone 2A5)

products short name :

Hsp27

products name syn :

Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.

other names :

heat shock protein beta-1; Heat shock protein beta-1; heat shock protein beta-1; 28 kDa heat shock protein; HSP 27; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; stress-responsive protein 27; heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

products gene name :

Hsp27

other gene names :

HSPB1; HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; HSP27; HSP28; HspB1; HSP 27; SRP27

uniprot entry name :

HSPB1_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

2A5

host :

Mouse

reactivity :

Human

sequence length :

205

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunohistochemistry (IHC)

app notes :

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

other info1 :

Antigen Species: Human. Clone: Anti-human Hsp27 mAb, clone 2A5, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp27 protein. Immunogen: Recombinant human Hsp27 (1-205aa) purified from E Coli

products categories :

Heat Shock Proteins

products description :

Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state.

products references :

Ehrnsperger M, et al., (1997) EMBO J 16:221-229 Steve J, et al., (2000) Mol Cell Biol 20(20):7602-7612 Butt E, et al., (2001) J Biol Chem 276:7108-7113 Michael, et al., (2002) Cell Stress Chsperones 7(3):281-296

ncbi gi num :

4504517

ncbi acc num :

NP_001531

ncbi gb acc num :

NM_001540.3

ncbi mol weight :

22,783 Da

ncbi pathways :

AUF1 (hnRNP D0) Destabilizes MRNA Pathway 187212!!Amoebiasis Pathway 167324!!Amoebiasis Pathway 167191!!Epstein-Barr Virus Infection Pathway 585562!!Epstein-Barr Virus Infection Pathway 587115!!FAS Pathway And Stress Induction Of HSP Regulation 198894!!Gene Expression Pathway 105937!!IL-3 Signaling Pathway 198881!!IL-6 Signaling Pathway 198864!!MAPK Signaling Pathway 198779

ncbi summary :

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008]

size :

0.05 ml

price :

240 USD

more info or order :

MyBioSource product webpage