Geminin antibody (clone 2H7) | MyBioSource MBS200034 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Geminin antibody (clone 2H7)

catalog :

MBS200034

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2H7

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200034

products type :

Antibody

products full name :

Geminin antibody (clone 2H7)

products short name :

Geminin

products name syn :

GMNN; Geminin; Geminin; DNA replication inhibitor; Gem; Geminin DNA replication inhibitor; OTTHuMP00000039393; RP3 369A17.3.

other names :

geminin; Geminin; geminin; geminin, DNA replication inhibitor

other gene names :

GMNN; GMNN; Gem

uniprot entry name :

GEMI_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

2H7

host :

Mouse

reactivity :

Human

sequence length :

209

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

other info1 :

Antigen Species: Human. Clone: Anti-human Geminin mAb, clone 2H7, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Geminin protein. Immunogen: Recombinant human Geminin (1-209 aa) purified from E Coli

products categories :

Signal Transduction

products description :

Geminin is a 25 kDa protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions.

products references :

McGarry TJ. et al., (1998) Cell. 93(6):1043-53. Pitulescu M. et al., (2005) Cell Mol Life Sci. 62(13):1425-33. Luo L. et al., (2004) Nature. 427(6976):749-53

ncbi gi num :

7705682

ncbi acc num :

NP_056979

ncbi gb acc num :

NM_015895.4

ncbi mol weight :

23,565 Da

ncbi pathways :

Assembly Of The Pre-replicative Complex Pathway (105821); Association Of Licensing Factors With The Pre-replicative Complex Pathway (105900); CDT1 Association With The CDC6:ORC:origin Complex Pathway (105824); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); DNA Replication Pathway (198771); DNA Replication Pathway (105897); DNA Replication Pre-Initiation Pathway (105898); M/G1 Transition Pathway (105820); Regulation Of DNA Replication Pathway (105899)

ncbi summary :

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

uniprot summary :

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family. Protein type: Motility/polarity/chemotaxis; Cell cycle regulation. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus. Molecular Function: protein binding; histone deacetylase binding; transcription corepressor activity. Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage