antibody

Park7/DJ-1 antibody (clone 1B11)

MBS200031

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

1B11

western blot, ELISA, enzyme immunoassay

Antibody

Park7/DJ-1 antibody (clone 1B11)

Park7/DJ-1

PARK7; DJ-1 (PARK7); Oncogene DJ1; Parkinson disease protein 7; Protein DJ-1; Parkinson disease protein 7 CAP1; DJ1; DJ1 protein; Oncogene DJ1; SP22.

protein DJ-1; Protein DJ-1; protein DJ-1; Parkinson disease (autosomal recessive, early onset) 7; epididymis secretory sperm binding protein Li 67p; oncogene DJ1; parkinson protein 7; Oncogene DJ1; Parkinson disease protein 7

Park7/DJ-1

PARK7; PARK7; DJ1; DJ-1; HEL-S-67p

PARK7_HUMAN

Monoclonal

IgG1, k

1B11

Mouse

Human

189

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Antigen Species: Human. Clone: Anti-human Park7/DJ-1 mAb, clone 1B11, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Park7/DJ-1 protein. Immunogen: Recombinant human Park7/DJ-1 (1-189 aa) purified from E Coli

Neuroscience

Park7/DJ-1, which is a small 189 amino acid protein, is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA-binding, androgen-receptor signaling and oxidative stress. Mutations in the Park7/DJ-1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (Park7).

Meulener MC. et al (2005) J. Neurochem. 93, 1524-1532 Bonifati V. et al (2003) Science. 299, 256-259 Zhou W. et al (2005) J. Biol. Chem. 280, 43150-43158 Junn E. et al (2005) PNAS. 102, 9691-9696

31543380

NP_009193

NM_007262.4

19,891 Da

Alpha-synuclein Signaling Pathway (137913); Androgen Receptor Signaling Pathway (198806); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Synaptic Vesicle Pathway (672457)

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration. Protein type: Tumor suppressor; Transcription regulation; Nuclear receptor co-regulator; EC 3.4.-.-. Chromosomal Location of Human Ortholog: 1p36.23. Cellular Component: PML body; mitochondrion; axon; mitochondrial matrix; cytoplasm; plasma membrane; mitochondrial intermembrane space; chromatin; nucleus; cytosol; mitochondrial respiratory chain complex I; lipid raft. Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; copper ion binding; enzyme binding; androgen receptor binding; peroxiredoxin activity; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding. Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; inflammatory response; negative regulation of protein binding; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of protein sumoylation; positive regulation of peptidyl-serine phosphorylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; lactate biosynthetic process; autophagy; negative regulation of protein kinase activity; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of apoptosis. Disease: Parkinson Disease 7, Autosomal Recessive Early-onset

0.05 mL

240 USD

0.1 mL

315