High Sensitive Bovine Serum Albumin (BSA) ELISA Kit | MyBioSource MBS2000307 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

High Sensitive Bovine Serum Albumin (BSA) ELISA Kit

catalog :

MBS2000307

quantity :

48-Strip-Wells

price :

430 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2000307

products type :

ELISA Kit

products full name :

High Sensitive Bovine Serum Albumin (BSA) ELISA Kit

products short name :

Bovine Serum Albumin (BSA)

products name syn :

Bovine Albumin; Fraction V

other names :

bovine serum albumin; Serum albumin; serum albumin; BSA; Allergen: Bos d 6

products gene name :

BSA

other gene names :

ALB; ALB

uniprot entry name :

ALBU_BOVIN

reactivity :

All

sequence length :

607

specificity :

This assay has high sensitivity and excellent specificity for detection of BSA. No significant cross-reactivity or interference between BSA and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Competitive. Detection Range: 246.9-20,000ng/mL. The standard curve concentrations used for the ELISA's were 20,000ng/mL, 6,666.7ng/mL, 2,222.2ng/mL, 740.7ng/mL, 246.9ng/mL. Sensitivity: Typically less than 87.2ng/mL

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BSA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BSA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Intended Uses The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of BSA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to BSA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled BSA and unlabeled BSA (Standards or samples) with the pre-coated antibody specific to BSA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of BSA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of BSA in the sample.

ncbi gi num :

3336842

ncbi acc num :

CAA76847.1

uniprot acc num :

P02769

ncbi mol weight :

69,293 Da

ncbi pathways :

Bile Acid And Bile Salt Metabolism Pathway (1120251); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (1119495); HDL-mediated Lipid Transport Pathway (1120218); Hemostasis Pathway (1120392); Lipid Digestion, Mobilization, And Transport Pathway (1120213); Lipoprotein Metabolism Pathway (1120216); Metabolism Pathway (1120205); Metabolism Of Lipids And Lipoproteins Pathway (1120212); Platelet Activation, Signaling And Aggregation Pathway (1120402); Platelet Degranulation Pathway (1120414)

uniprot summary :

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Protein type: Secreted, signal peptide; Secreted; Carrier. Cellular Component: protein complex; cytoplasm; extracellular region; nucleus. Molecular Function: toxin binding; DNA binding; metal ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding. Biological Process: retinal homeostasis; maintenance of mitochondrion localization; transport; hemolysis by symbiont of host red blood cells; cellular response to starvation; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

430 USD

size2 :

96-Strip-Wells

price2 :

575

size3 :

5x96-Strip-Wells

price3 :

2175

size4 :

10x96-Strip-Wells

price4 :

3890

more info or order :

MyBioSource product webpage