ELISA/assay

Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit

MBS2000305

24-Strip-Wells

270 USD

ELISA Kit

Regenerating Islet Derived Protein 3 Beta (REG3b) ELISA Kit

[Regenerating Islet Derived Protein 3 Beta (REG3b)]

[PAP1; Pancreatitis-Associated Protein 1]

[regenerating islet-derived protein 3-beta; Regenerating islet-derived protein 3-beta; regenerating islet-derived protein 3-beta; regenerating islet-derived 3 beta; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-beta; Reg III-beta]

[REG3b]

[Reg3b; Reg3b; HIP; Pap; PAP1; REG-III; Pap; Pap1; REG-3-beta; Reg III-beta]

REG3B_MOUSE

Mouse

175

This assay has high sensitivity and excellent specificity for detection of REG1b. No significant cross-reactivity or interference between REG1b and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Assay Type: Quantitative Sandwich. Detection Range: 1.56-100ng/mL. Sensitivity: 0.62ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level REG1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level REG1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Human gene deletion

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REG1b in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to REG1b. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to REG1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of REG1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6754980

NP_035166.1

NM_011036.1

P35230

19,476 Da

REG3G: Might be a stress protein involved in the control of bacterial proliferation. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: carbohydrate binding. Biological Process: defense response to Gram-positive bacterium; acute-phase response; inflammatory response; defense response to Gram-negative bacterium

24-Strip-Wells

270 USD

48-Strip-Wells

505

96-Strip-Wells

685

5x96-Strip-Wells

2770

10x96-Strip-Wells

5025