ELISA/assay

Inositol Triphosphate (IP3) ELISA Kit

MBS2000302

48-Strip-Wells

640 USD

ELISA Kit

Inositol Triphosphate (IP3) ELISA Kit

Inositol Triphosphate (IP3)

ELISA Kit for Inositol Triphosphate (IP3)

IP3

Most species

This assay has high sensitivity and excellent specificity for detection of IP3. No significant cross-reactivity or interference between IP3 and analogues was observed.

For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Assay Type: Competitive. Detection Range: 123.5-10,000pg/mL. The standard curve concentrations used for the ELISA's were 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, 123.5pg/mL. Sensitivity: Typically less than 52.5pg/mL

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IP3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IP3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intended Uses The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IP3 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IP3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IP3 and unlabeled IP3 (Standards or samples) with the pre-coated antibody specific to IP3. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IP3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IP3 in the sample.

48-Strip-Wells

640 USD

96-Strip-Wells

875

5x96-Strip-Wells

3455

10x96-Strip-Wells

6170