Alpha-Crystallin B antibody (clone 2E8) | MyBioSource MBS200030 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Alpha-Crystallin B antibody (clone 2E8)

catalog :

MBS200030

quantity :

0.05 ml

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2.00E+08

reactivity :

human, mouse

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200030

products type :

Antibody

products full name :

Alpha-Crystallin B antibody (clone 2E8)

products short name :

Alpha-Crystallin B

products name syn :

Crya2; Crya-2; Cryab; Crystallin alpha B

other names :

alpha-crystallin B chain; Alpha-crystallin B chain; alpha-crystallin B chain; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; renal carcinoma antigen NY-REN-27; rosenthal fiber component; crystallin, alpha B; Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component

other gene names :

CRYAB; CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101; CRYA2; HspB5

uniprot entry name :

CRYAB_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, k

clone :

2.00E+08

host :

Mouse

reactivity :

Human, Mouse

sequence length :

175

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000. Please note that this antibody (2E8) has the specificity against crystallin alpha B, do not detect crystallin alpha A.

other info1 :

Antigen Species: Human. Clone: Anti-human Crystallin alpha B mAb, clone 2E8, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Crystallin alpha B. Immunogen: Recombinant human Crystallin alpha B (1-175 aa) purified from E Coli

products categories :

Heat Shock Proteins

products description :

alpha-Crystallins are composed of two gene products; alpha-Crystallin A and B, for acidic and basic, respectively. alpha-Crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-Crystallin A and B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. alpha-Crystallin B is expressed widely in many tissues and organs and occurs in many neurological diseases.

products references :

Hasan, A., et al. (2002) Biochemistry 41(52) 15876-15882 kamradt,M.C., et al (2002) J. Biol. Chem. 277(41) 38731-38736 Reddy, G.B., et al (2002) FEBS lett. 522(1-3) 59-64 Ito, H., et al (2002) J. Biochem. 131(4) 593-603

ncbi gi num :

4503057

ncbi acc num :

NP_001876.1

ncbi gb acc num :

NM_001885.2

ncbi mol weight :

20,159 Da

ncbi pathways :

Protein Processing In Endoplasmic Reticulum Pathway 167325!!Protein Processing In Endoplasmic Reticulum Pathway 167190

ncbi summary :

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

size :

0.05 ml

price :

240 USD

more info or order :

MyBioSource product webpage