ELISA/assay

Indole 3 Acetic Acid (IAA) ELISA Kit

MBS2000290

24-Strip-Wells

290 USD

ELISA Kit

Indole 3 Acetic Acid (IAA) ELISA Kit

[Indole 3 Acetic Acid (IAA)]

[IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin]

[IAA]

General

This assay has high sensitivity and excellent specificity for detection of hyaluronic acid. No significant cross-reactivity or interference between hyaluronic acid and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 4.94-400ng/mL. Sensitivity: 1.81ng/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level hyaluronic acid were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level hyaluronic acid were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction; Hormone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of hyaluronic acid in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to hyaluronic acid has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled hyaluronic acid and unlabeled hyaluronic acid (Standards or samples) with the pre-coated antibody specific to hyaluronic acid. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of hyaluronic acid in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of hyaluronic acid in the sample.

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

745

5x96-Strip-Wells

3035

10x96-Strip-Wells

5475