antibody

HtrA2/Omi antibody (clone 1B3)

MBS200025

0.05 ml

240 USD

monoclonal

mouse

nonconjugated

1B3

human, mouse

western blot, ELISA, enzyme immunoassay

Antibody

HtrA2/Omi antibody (clone 1B3)

HtrA2/Omi

HTRA2; OMI; PRSS25; HtrA2/Omi; EC 3.4.21.108; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; Serine protease HTRA2; mitochondrial; High temperature requirement protein A2

serine protease HTRA2, mitochondrial isoform 1 preproprotein; Serine protease HTRA2, mitochondrial; serine protease HTRA2, mitochondrial; HtrA-like serine protease; Omi stress-regulated endoprotease; high temperature requirement protein A2; protease, serine, 25; serine protease 25; serine proteinase OMI; HtrA serine peptidase 2; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI

HtrA2/Omi

HTRA2; HTRA2; OMI; PARK13; PRSS25; OMI; PRSS25; HtrA2

HTRA2_HUMAN

Monoclonal

IgG1, k

1B3

Mouse

Human, Mouse

458

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.

Antigen Species: Human. Clone: Anti-human HtrA2/Omi mAb, clone 1B3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HtrA2/Omi protein. Immunogen: Recombinant human HtrA2/Omi (134-458aa) purified from E Coli

Cancer

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death

Miller L., et al., (1999) Trends Cell Biol. 9:323-28 Zumbrunn J., et al., (1996) FABS Lett. 398:187-92 Gui-Ying N., et al., (2003) Biochem. J. 371:39-48

7019477

NP_037379

NM_013247.4

39,914 Da

Apoptosis Modulation And Signaling Pathway 198822!!Parkinson's Disease Pathway 83098!!Parkinsons Disease Pathway 705377

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008]

0.05 ml

240 USD