ELISA/assay

Gibberellic Acid (GA) ELISA Kit

MBS2000244

96 Strip Wells

695 USD

ELISA Kit

Gibberellic Acid (GA) ELISA Kit

Gibberellic Acid (GA)

GA3; Gibberellin A3; Gibberellins

GA

All

This assay has high sensitivity and excellent specificity for detection of Gibberellic Acid (GA). No significant cross-reactivity or interference between Gibberellic Acid (GA) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Sample Types: Tissue or cell culture supernates. Detection Range: 123.46-10000ng/mL. Sensitivity: The minimum detectable dose of this kit is typically less than 42.9ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gibberellic Acid (GA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C;. 3. Aspirate and wash 3 times;. 4. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 5. Aspirate and wash 5 times;. 6. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 7. Add 50uL Stop Solution. Read at 450 nm immediately.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Gibberellic Acid (GA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Gibberellic Acid (GA) and unlabeled Gibberellic Acid (GA) (Standards or samples) with the pre-coated antibody specific to Gibberellic Acid (GA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Gibberellic Acid (GA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Gibberellic Acid (GA) in the sample.

96 Strip Wells

695 USD