ELISA/assay

Histamine (HA) ELISA Kit

MBS2000219

24-Strip-Wells

290 USD

ELISA Kit

Histamine (HA) ELISA Kit

[Histamine (HA)]

[Histamin]

[HA]

General

This assay has high sensitivity and excellent specificity for detection of Histamine (HA). No significant cross-reactivity or interference between Histamine (HA) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Standard Curve/Testing Data

Assay Type: Competitive Inhibition. Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids. Detection Range: 1.23-100ng/mL. Sensitivity: < 0.52ng/mL

Application: Enzyme-linked immunosorbent assay for Antigen Detection. Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histamine (HA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histamine (HA) were tested on 3 different plates, 8 replicates in each plate. CV(%) =: SD/meanX100. Intra-Assay: CV<10%. Inter-Assay: CV<12%

Signal transduction; Infection immunity

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Histamine (HA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Histamine (HA) and unlabeled Histamine (HA) (Standards or samples) with the pre-coated antibody specific to Histamine (HA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Histamine (HA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Histamine (HA) in the sample.

24-Strip-Wells

290 USD

48-Strip-Wells

550

96-Strip-Wells

745

5x96-Strip-Wells

3035

10x96-Strip-Wells

5475