Sulfatase 2 (SULF2) ELISA Kit | MyBioSource MBS2000204 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Sulfatase 2 (SULF2) ELISA Kit

catalog :

MBS2000204

quantity :

48-Strip-Wells

price :

575 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2000204

products type :

ELISA Kit

products full name :

Sulfatase 2 (SULF2) ELISA Kit

products short name :

Sulfatase 2 (SULF2)

products name syn :

HSULF-2; Extracellular sulfatase Sulf-2

other names :

Sulfatase 2; Extracellular sulfatase Sulf-2; extracellular sulfatase Sulf-2; sulfatase 2

products gene name :

SULF2

other gene names :

SULF2; SULF2; HSULF-2; KIAA1247; hSulf-2

uniprot entry name :

SULF2_HUMAN

reactivity :

Mouse

sequence length :

870

specificity :

This assay has high sensitivity and excellent specificity for detection of Sulfatase 2 (SULF2). No significant cross-reactivity or interference between Sulfatase 2 (SULF2) and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/mL. Sensitivity: Typically less than 13.2pg/mL.

other info2 :

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

products description :

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfatase 2 (SULF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfatase 2 (SULF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfatase 2 (SULF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Sulfatase 2 (SULF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

83405533

ncbi acc num :

AAI10540.1

uniprot acc num :

Q8IWU5

ncbi mol weight :

100,156 Da

ncbi summary :

Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]

uniprot summary :

SULF2: Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Belongs to the sulfatase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.1.6.-; Hydrolase; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space; Golgi stack; cell surface; endoplasmic reticulum; plasma membrane. Molecular Function: arylsulfatase activity; N-acetylglucosamine-6-sulfatase activity; calcium ion binding. Biological Process: negative regulation of fibroblast growth factor receptor signaling pathway; embryonic skeletal development; cartilage development; glomerular basement membrane development; glomerular filtration; esophagus smooth muscle contraction; heparan sulfate proteoglycan metabolic process; chondrocyte development; kidney development; positive regulation of Wnt receptor signaling pathway

size1 :

48-Strip-Wells

price1 :

575 USD

size2 :

96-Strip-Wells

price2 :

785

size3 :

5x96-Strip-Wells

price3 :

3055

size4 :

10x96-Strip-Wells

price4 :

5495

more info or order :

MyBioSource product webpage