ELISA/assay

Coxsackie Virus And Adenovirus Receptor (CXADR) ELISA Kit

MBS2000196

48-Strip-Wells

565 USD

ELISA Kit

Coxsackie Virus And Adenovirus Receptor (CXADR) ELISA Kit

Coxsackie Virus And Adenovirus Receptor (CXADR)

CAR; HCAR; HCVADR; CVB3-binding protein; Coxsackievirus B-adenovirus receptor

Coxsackie virus and adenovirus receptor; Coxsackievirus and adenovirus receptor; coxsackievirus and adenovirus receptor; coxsackie virus and adenovirus receptor; CVB3-binding protein; Coxsackievirus B-adenovirus receptor; HCVADR

CXADR

CXADR; CXADR; CAR; HCAR; CAR4/6; CAR; CAR; hCAR

CXAR_HUMAN

Human

365

This assay has high sensitivity and excellent specificity for detection of Coxsackie Virus And Adenovirus Receptor (CXADR). No significant cross-reactivity or interference between Coxsackie Virus And Adenovirus Receptor (CXADR) and analogues was observed.

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Samples: Tissue homogenates, Cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. Sensitivity: Typically less than 0.054ng/mL.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coxsackie Virus And Adenovirus Receptor (CXADR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coxsackie Virus And Adenovirus Receptor (CXADR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 . Intra-Assay: CV<10% . Inter-Assay: CV<12% . Assay Procedure Summary: 1. Prepare all reagents, samples and standards;. 2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;. 3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;. 4. Aspirate and wash 3 times;. 5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;. 6. Aspirate and wash 5 times;. 7. Add 90uL Substrate Solution. Incubate 15-25 minutes at 37 degree C;. 8. Add 50uL Stop Solution. Read at 450nm immediately.

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coxsackie Virus And Adenovirus Receptor (CXADR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coxsackie Virus And Adenovirus Receptor (CXADR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coxsackie Virus And Adenovirus Receptor (CXADR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Coxsackie Virus And Adenovirus Receptor (CXADR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

14714775

AAH10536.1

P78310

35,570 Da

Adaptive Immune System Pathway (366160); Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413); Viral Myocarditis Pathway (125138)

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN. Protein type: Receptor, misc.; Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 21q21.1. Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; apicolateral plasma membrane; basolateral plasma membrane; extracellular region; acrosome; intercellular junction; lipid raft; nucleoplasm; growth cone; adherens junction; plasma membrane; cell junction; neuromuscular junction; filopodium. Molecular Function: viral receptor activity; integrin binding; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; receptor binding; PDZ domain binding. Biological Process: mitochondrion organization and biogenesis; intercellular junction assembly and maintenance; neutrophil chemotaxis; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; homotypic cell-cell adhesion; germ cell migration; actin cytoskeleton reorganization; blood coagulation; leukocyte migration; defense response to virus

48-Strip-Wells

565 USD

96-Strip-Wells

765

5x96-Strip-Wells

2975

10x96-Strip-Wells

5340