catalog number :
MBS2000136
products type :
ELISA Kit
products full name :
Lipopolysaccharide (LPS) ELISA Kit
products short name :
[Lipopolysaccharide (LPS)]
products name syn :
[LOS; Lipoglycans; Lipooligosaccharide; Lipo-Oligosaccharide; Endotoxin]
other names :
[lipopolysaccharide; Lipopolysaccharide-processing protein LpsZ; Polysaccharide chain length determinant protein]
products gene name :
[LPS]
other gene names :
[lpsZ; rkpZ]
uniprot entry name :
LPSZ_RHIML
specificity :
This assay has high sensitivity and excellent specificity for detection of LH. No significant cross-reactivity or interference between LH and analogues was observed.
storage stability :
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Rat Serum, Plasma Or Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 0.468-30ng/mL. Sensitivity: 0.14ng/mL.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
products description :
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of LH in rat serum, plasma or other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to LH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled LH and unlabeled LH (Standards or samples) with the pre-coated antibody specific to LH. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of LH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of LH in the sample.
ncbi acc num :
AAA26297.1
ncbi mol weight :
48,590 Da
uniprot summary :
Involved in the invasion of nitrogen fixation nodules. May be involved in the biosynthesis of lipopolysaccharides as an enzyme or a regulatory protein.
size5 :
10x96-Strip-Wells