antibody

RASSF1A antibody (clone 3F3)

MBS200009

0.05 ml

240 USD

monoclonal

mouse

nonconjugated

3F3

western blot, ELISA, immunocytochemistry, enzyme immunoassay

Antibody

RASSF1A antibody (clone 3F3)

RASSF1A

RASSF1(Ras association (RalGDS/AF-6) domain family 1); 123F2; RDA32; NORE2A; RASSF1A; REH3P21; Ras association (RalGDS/AF-6) domain family member 1

ras association domain-containing protein 1 isoform A; Ras association domain-containing protein 1; ras association domain-containing protein 1; RASSF1; WUGSC:H_LUCA12.5; cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; tumor suppressor protein RDA32; Ras association (RalGDS/AF-6) domain family member 1

RASSF1A

RASSF1; RASSF1; 123F2; RDA32; NORE2A; RASSF1A; REH3P21

RASF1_HUMAN

Monoclonal

IgG1, k

3F3

Mouse

Human

340

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Cell lysate), Immunofluorescence (IF)

The antibody has been tested by ELISA, Western blot and immunocytochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2,000. Recommended starting dilution is 1:1,000.

Antigen Species: Human. Clone: Anti-human RASSF1A mAb, clone 3F3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant RASSF1A. Immunogen: Recombinant human RASSF1A (1-340aa) purified from E Coli

Cancer

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.

Agathanggelou A, et al., (2005) Cancer Res. 65, 3497-3508 Lerman MI. et. al. (2000) Cancer Res. 60, 6116-6133 Strunnikova M. et al., (2005) Mol Cell Biol. 25, 3923-3933 Dammann R. et al., (2005) Histol Histopathol. 20, 645-663 Rossa W.k, et al., (2007) American Journal of pathology. 170, 941-950

20149588

NP_009113

NM_007182.4

8,656 Da

Bladder Cancer Pathway 83115!!Bladder Cancer Pathway 527!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!Non-small Cell Lung Cancer Pathway 83119!!Non-small Cell Lung Cancer Pathway 531!!Pathways In Cancer 83105!!Ras Signaling Pathway 868085

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]

0.05 ml

240 USD