ELISA/assay

Gamma-Aminobutyric Acid (gABA) ELISA Kit

MBS2000082

24-Strip-Wells

265 USD

ELISA Kit

Gamma-Aminobutyric Acid (gABA) ELISA Kit

[Gamma-Aminobutyric Acid (gABA)]

[4-Aminobutyric Acid]

[gamma-aminobutyric acid]

[gABA]

General

462

This assay has high sensitivity and excellent specificity for detection of fT3. No significant cross-reactivity or interference between fT3 and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma And Other Biological Fluids. Assay Type: Quantitative Competitive. Detection Range: 740.7-60,000fg/mL. Sensitivity: 254.1fg/mL.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level fT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level fT3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Signal transduction; Cardiovascular biology; Neuroscience; Bone metabolism

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of fT3 in serum, plasma and other biological fluids. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to T3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled T3 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of fT3 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of fT3 in the sample.

60654715

AAX31922.1

24-Strip-Wells

265 USD

48-Strip-Wells

480

96-Strip-Wells

645

5x96-Strip-Wells

2620

10x96-Strip-Wells

4725