antibody

Growth Hormone Antibody (clone g3H5)

MBS200008

0.05 mL

240 USD

monoclonal

mouse

nonconjugated

g3H5

western blot, ELISA, enzyme immunoassay

Antibody

Growth Hormone Antibody (clone g3H5)

Growth Hormone

GH1; Growth Hormone; GH; GH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin; GHN; hGH-N

somatotropin isoform 1; Somatotropin; somatotropin; pituitary growth hormone; growth hormone 1; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone

GH1; GH1; GH; GHN; GH-N; hGH-N; IGHD1B; GH; GH-N

SOMA_HUMAN

Monoclonal

IgG2b, k

g3H5

Mouse

Human, Mouse

217

By protein-G affinity chromatography

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

1mg/ml

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

ELISA (EIA), Western Blot (WB) (Recombinant protein)

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Antigen Species: Human. Clone: Anti-human growth hormone mAb, clone g3H5, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant growth hormone. Immunogen: Recombinant human growth hormone (27-217aa) purified from E Coli

Growth factors

Human growth hormone(hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.

Wickens, et al., (1978) J. Biol Chem. 253(7)2483-2495 Chang, et al., (1978) Nature. 275,617-624 Ullrich. et al., (1977) Science 196, 1313-1319. Tsushima, et al., (2000) Endocr J. 47; S17-21 Horan, M. et al.,(2003) Hum. Mutat.21(4), 408-423

13027812

NP_000506

NM_000515.4

20,201 Da

Adipogenesis Pathway (198832); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (106230)

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

GH: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A); also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH. Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH. Defects in GH1 are the cause of Kowarski syndrome (KWKS); also known as pituitary dwarfism VI. Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2). IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy. Belongs to the somatotropin/prolactin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Hormone. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; growth hormone receptor binding; growth factor activity; prolactin receptor binding; metal ion binding; hormone activity. Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of receptor internalization; positive regulation of JAK-STAT cascade; glucose transport; positive regulation of multicellular organism growth; JAK-STAT cascade; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein. Disease: Isolated Growth Hormone Deficiency, Type Ia; Isolated Growth Hormone Deficiency, Type Ib; Isolated Growth Hormone Deficiency, Type Ii; Kowarski Syndrome

0.05 mL

240 USD

0.1 mL

315