Divalent Metal Transporter 1 (DMT1) ELISA Kit | MyBioSource MBS2000079 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Divalent Metal Transporter 1 (DMT1) ELISA Kit

catalog :

MBS2000079

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2000079

products type :

ELISA Kit

products full name :

Divalent Metal Transporter 1 (DMT1) ELISA Kit

products short name :

Divalent Metal Transporter 1 (DMT1)

products name syn :

SLC11A2; DCT1; NRAMP2; Natural resistance-associated macrophage protein 2; Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters

other names :

divalent metal transporter 1; Divalent metal transporter 1; natural resistance-associated macrophage protein 2; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2; Divalent metal transporter 1

products gene name :

DMT1

other gene names :

slc11a2; slc11a2; DMT1; cb426; fa07b10; wu:fa07b10; zgc:136699

uniprot entry name :

Q8AX90_DANRE

reactivity :

Human

sequence length :

547

specificity :

This assay has high sensitivity and excellent specificity for detection of DMT1. No significant cross-reactivity or interference between DMT1 and analogues was observed.

storage stability :

For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C upon receipt while the others should be at 4 degree C. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal. The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

other info1 :

Samples: Human tissue homogenates, cell lysates and other biological fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/mL. The standard curve concentrations used for the ELISA's were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL. Sensitivity: The minimum detectable dose of DMT1 is typically less than 0.064ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

other info2 :

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DMT1 in human tissue homogenates, cell lysates and other biological fluids. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DMT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DMT1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DMT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DMT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DMT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DMT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

27475929

ncbi acc num :

AAO16988.1

ncbi mol weight :

60,107 Da

ncbi pathways :

Iron Uptake And Transport Pathway (1114051); Lysosome Pathway (98649); Lysosome Pathway (96865); Metal Ion SLC Transporters Pathway (1114033); SLC-mediated Transmembrane Transport Pathway (1114010); Transmembrane Transport Of Small Molecules Pathway (1114006); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1114026)

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

765

size3 :

5x96-Strip-Wells

price3 :

2975

size4 :

10x96-Strip-Wells

price4 :

5340

more info or order :

MyBioSource product webpage