A crystallin A antibody (clone c9F2) | MyBioSource MBS200007 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

A crystallin A antibody (clone c9F2)

catalog :

MBS200007

quantity :

0.05 mL

price :

240 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

c9F2

reactivity :

human, rat

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS200007

products type :

Antibody

products full name :

A crystallin A antibody (clone c9F2)

products short name :

A crystallin A

products name syn :

Heat shock protein beta-4; HspB4; Alpha-crystallin A chain; Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; CRYA1; CRYAA; Crystallin Alpha 1; Crystallin alpha A; HSPB 4; Zonular Central Nuclear Cataract

other names :

alpha-crystallin A chain; Alpha-crystallin A chain; alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1); crystallin, alpha A; Heat shock protein beta-4; HspB4

other gene names :

CRYAA; CRYAA; CRYA1; HSPB4; CTRCT9; CRYA1; HSPB4; HspB4

uniprot entry name :

CRYAA_HUMAN

clonality :

Monoclonal

isotype :

IgG1, k

clone :

c9F2

host :

Mouse

reactivity :

Human, Rat

sequence length :

173

purity :

By protein-G affinity chromatography

form :

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide.

concentration :

1mg/ml

storage stability :

Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

tested application :

ELISA (EIA), Western Blot (WB) (Cell lysate)

app notes :

The antibody has been tested by ELISA and by Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis: 1:500 ~ 1:2000. Recommended starting dilution : 1:1000.

other info1 :

Antigen Species: Human. Clone: Anti-human Crystallin alpha A mAb, clone c9F2, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant Crystallin alpha A. Immunogen: Recombinant human Crystallin alpha A (1-173aa) purified from E Coli

products categories :

Heat Shock Proteins

products description :

alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and the participation in the intracellular architecture.

products references :

Fujii N., et al., (2003) Mol. Vis. 9: 915-22 Laksanalamai P., et al., (2004) Extremophiles 8: 1-11

ncbi gi num :

4503055

ncbi acc num :

NP_000385

ncbi gb acc num :

NM_000394.3

ncbi mol weight :

19,909 Da

ncbi pathways :

Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

uniprot summary :

CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens. Protein type: Heat shock protein; Chaperone. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: cytoplasm; nucleus. Molecular Function: identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens. Biological Process: visual perception; negative regulation of intracellular transport; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis. Disease: Cataract 9, Multiple Types

size1 :

0.05 mL

price1 :

240 USD

size2 :

0.1 mL

price2 :

315

more info or order :

MyBioSource product webpage